Basic principles of plastid transformation:
First, the gene or genes to be introduced into the plastid genome are coated onto microscopic gold particles (0.6-1 ìm in diameter). These DNA-coated gold-particles are then shot into plant cells using a helium-driven biolistic gun. Following shooting, transformed plant cells (plant cells that contain a plastid or plastids with the gene of interest) are selected and a new transplastomic plant is regenerated from this plant cell. Although simple in principle, the selection and regeneration of transplastomic plants is prone to errors using current conventional antibiotics-based selection methods. We have developed a new selection and regeneration method which is more efficient and reliable compared to currently available technologies.
Method patent 1: WO/2008/071973 - granted in GB, pending in EU, US, Canada, Japan, Norway.
Method patent 2: WO/2009/150442 - pending in EU, US, Canada, Brazil, China, Australia, India.
UK Priority patent Application No. 1413897.8 /
US Provisional Patent Application No. 61/981386
Med revolusjonerende metoder skal selskapet Plastid ASprodusere proteiner. Professor Simon Geir Møller står i spissen for selskapet, som er Universitetet i Stavangers første biotek-selskap.
Photos: Håkon Vold