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Technology

 

Basic principles of plastid transformation:

First, the gene or genes to be introduced into the plastid genome are coated onto microscopic gold particles (0.6-1 ìm in diameter). These DNA-coated gold-particles are then shot into plant cells using a helium-driven biolistic gun. Following shooting, transformed plant cells (plant cells that contain a plastid or plastids with the gene of interest) are selected and a new transplastomic plant is regenerated from this plant cell. Although simple in principle, the selection and regeneration of transplastomic plants is prone to errors using current conventional antibiotics-based selection methods. We have developed a new selection and regeneration method which is more efficient and reliable compared to currently available technologies.

  • Positive selection
  • Proven potential of the selectable marker in tomato, 
lettuce and maize
  • Homologous recombination - accurate targeting into 
chloroplast genome
  • Expression of multiple genes from polycistronic mRNA
  • Epigenetic effects (silencing) absent
  • Not based on antibiotics
  • High expression levels (~10-20% tpc)
  • No transgene spread through pollen

Method patent 1: WO/2008/071973 - granted in GB, pending in EU, US, Canada, Japan, Norway.

 

Method patent 2: WO/2009/150442 - pending in EU, US, Canada, Brazil, China, Australia, India.

 

UK Priority patent Application No. 1413897.8 /

US Provisional Patent Application No. 61/981386

 

 

Livreddende biotek ved UiS

 

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Med revolusjonerende metoder skal selskapet Plastid ASprodusere proteiner. Professor Simon Geir Møller står i spissen for selskapet, som er Universitetet i Stavangers første biotek-selskap.

Photos: Håkon Vold